Patient Guide

Complete Guide to Anaemia

Anaemia is not a single diagnosis — it is a symptom with many causes. Identifying the type of anaemia through blood tests is essential before starting any treatment.

What is anaemia?

Anaemia is defined as haemoglobin below the normal range for sex and age: below 13.5 g/dL in adult men, below 12.0 g/dL in adult women, and below 11.0 g/dL in pregnant women. It affects approximately 1.6 billion people globally — making it one of the most common medical conditions. The cause determines the treatment, which is why a systematic approach using blood tests is essential.

Step 1: CBC + blood film — classifying anaemia by red cell size

MCV (mean cell volume)Red cell appearanceLikely cause
Low MCV (<80 fL)Microcytic (small cells)Iron deficiency (most common), thalassaemia, sideroblastic anaemia
Normal MCV (80–100 fL)Normocytic (normal size)Anaemia of chronic disease, haemolysis, blood loss (acute), kidney disease
High MCV (>100 fL)Macrocytic (large cells)Vitamin B12 deficiency, folate deficiency, alcohol, hypothyroidism, liver disease

Step 2: Iron deficiency anaemia workup

TestIron deficiency finding
Ferritin<30 ng/mL (often much lower) — most sensitive marker
Serum ironLow
TIBCHigh (body compensates by making more transport protein)
Transferrin saturation<16%
MCVLow (<80 fL)
Blood filmMicrocytic, hypochromic cells; target cells

Step 3: Megaloblastic anaemia workup

TestB12 or folate deficiency finding
Serum B12<200 pg/mL (deficiency); <100 (severe)
Serum folate<3.0 ng/mL
MCV>100 fL (macrocytic)
Blood filmOval macrocytes; hypersegmented neutrophils (5+ lobes)
Peripheral blood smearHypersegmented neutrophils are pathognomonic of megaloblastic anaemia

Step 4: Haemolytic anaemia workup

TestFinding in haemolysis
LDHElevated (released from lysed red cells)
Indirect bilirubinElevated (breakdown product of haemoglobin)
HaptoglobinLow or absent (mops up free haemoglobin; depleted in haemolysis)
Reticulocyte countHigh (bone marrow compensating)
Blood filmSpherocytes (hereditary spherocytosis), sickle cells, fragmented cells (microangiopathy)
Direct Coombs testPositive in autoimmune haemolytic anaemia

Common treatments by anaemia type

TypeTreatment
Iron deficiencyFerrous sulfate oral iron (200 mg 2-3x daily) or IV iron; identify and treat source of blood loss
Vitamin B12 deficiencyIM cyanocobalamin/methylcobalamin injections; or high-dose oral B12 (1,000 mcg daily)
Folate deficiencyFolic acid 5 mg daily for 4 months; address underlying cause
Anaemia of chronic diseaseTreat underlying condition; EPO injections for kidney disease-associated anaemia
Thalassaemia (minor)Usually no treatment needed; genetic counselling

Questions to ask your doctor

Medical Disclaimer: This page is for general educational purposes only and does not constitute medical advice. Always consult a qualified doctor for diagnosis and treatment.