Blood Test Guide

ANA Test (Antinuclear Antibody)

A positive ANA test is common but not always serious. It can indicate lupus, Sjögren's or other autoimmune conditions — but also occurs in healthy people. Here's what to know.

ANA test results interpretation

ResultInterpretation
Negative / <1:40Normal — autoimmune disease very unlikely
1:40 to 1:80Low positive — weakly positive, found in 20–30% of healthy people
1:160 to 1:320Moderate positive — more significant, further testing warranted
≥1:640High positive — strongly suggests autoimmune disease

What does a positive ANA mean?

Positive ANA is common — but not always disease

ANA is positive in about 95% of people with lupus (SLE), but also in 5–15% of completely healthy people — especially women and older adults. A positive ANA alone does not diagnose any disease. The titre (level) and pattern (speckled, homogeneous, nucleolar) help direct further testing. Further specific antibodies are tested when ANA is significantly positive: anti-dsDNA and anti-Sm for lupus; anti-Ro/La for Sjögren's; anti-Scl-70 for scleroderma; anti-Jo-1 for myositis.

Conditions associated with positive ANA

ConditionANA positivity rate
Systemic Lupus Erythematosus (SLE)~95%
Sjögren's syndrome~80%
Scleroderma~80%
Rheumatoid arthritis~30–40%
Healthy individuals5–15%

Questions to ask your doctor

Medical Disclaimer: For educational purposes only. Always consult a qualified healthcare professional for diagnosis and treatment.