Hormone Test

Cortisol Blood Test

Cortisol is the body's main stress hormone, produced by the adrenal glands. It regulates metabolism, blood pressure and immune function. Too much or too little causes serious conditions including Cushing syndrome and Addison disease.

What is cortisol?

Cortisol is a steroid hormone produced by the adrenal cortex (the outer layer of the adrenal glands, which sit on top of the kidneys). It follows a strong diurnal (daily) rhythm — highest in the early morning (peaking around 8am) and lowest at midnight. Cortisol regulates: blood sugar, blood pressure, anti-inflammatory response, metabolism of fats/proteins/carbohydrates, and the body's response to stress. Cortisol testing must be interpreted carefully in the context of the time of day and clinical situation.

Cortisol normal range

TimeNormal Range (mcg/dL)Notes
Morning (8am)10 – 20Peak; best time for testing
Afternoon (4pm)3 – 10Falls through the day
Evening / midnight< 3Near baseline
RandomVaries widelyTime of sample must be noted

HIGH Cortisol — Cushing Syndrome

Persistently elevated cortisol is called Cushing syndrome. Causes: long-term steroid medication (most common), a pituitary tumour producing excess ACTH (Cushing disease), an adrenal tumour producing excess cortisol, or an ectopic ACTH-producing tumour (lung, pancreas). Symptoms: central obesity (round face and belly with thin limbs), purple stretch marks (striae), easy bruising, high blood pressure, high blood sugar, muscle weakness, depression, and in women — irregular periods and hirsutism. Diagnosis involves 24-hour urine cortisol, late-night salivary cortisol and a 1mg dexamethasone suppression test.

LOW Cortisol — Adrenal Insufficiency

Low cortisol is called adrenal insufficiency. Primary adrenal insufficiency (Addison disease) means the adrenal glands are damaged — causes include autoimmune destruction (most common in developed countries), tuberculosis, HIV/AIDS, cancer and bilateral adrenal haemorrhage. Secondary adrenal insufficiency means the pituitary is not producing enough ACTH — most commonly caused by sudden withdrawal of long-term steroid therapy. Symptoms: profound fatigue, weight loss, low blood pressure, salt craving, nausea, darkening of the skin (primary only). An adrenal crisis (acute severe cortisol deficiency) is a medical emergency.

Questions to ask your doctor

Medical Disclaimer: This page is for general educational purposes only and does not constitute medical advice. Always consult a qualified doctor for diagnosis and treatment decisions.