Lab Test

Folate Test: B9 Deficiency, Pregnancy & Anaemia

Folate deficiency causes megaloblastic anaemia and — critically in pregnancy — neural tube defects. It's preventable with supplementation and a varied diet.

Normal serum folate
>7 nmol/L
Red cell folate (better store)
>340 nmol/L
Pregnancy supplement
400 μg/day pre-conception
Neural tube defect risk
Reduced by 70% with supplementation

Folate vs B12

FeatureFolateVitamin B12
RoleDNA synthesis, cell divisionDNA synthesis + nerve myelin + homocysteine
Anaemia typeMegaloblastic (high MCV)Megaloblastic (high MCV)
Nerve damageNoYes — subacute combined degeneration
StoresWeeks–months (depleted faster)Years (large hepatic stores)
Pregnancy importanceCritical — neural tube defectsAlso important
Main food sourcesGreen leafy veg, legumes, fortified foodsAnimal products only

Causes of Folate Deficiency

Red Cell Folate is More ReliableSerum folate reflects recent dietary intake and can be transiently normal after a single meal. Red cell folate better reflects tissue stores over the preceding 2–3 months — request both.
When should I take folic acid in pregnancy?
Start folic acid (400 μg/day) at least 1 month before conception and continue until 12 weeks. Women with epilepsy, diabetes, obesity (BMI>30), or previous NTD-affected pregnancy should take 5 mg/day.
Can folate deficiency cause depression?
Low folate is associated with depression and poor antidepressant response. Augmenting antidepressants with folic acid (methylfolate) has evidence in some patients.
What is the difference between folate and folic acid?
Folic acid is the synthetic, oxidised form used in supplements and food fortification. Folate is the naturally occurring reduced form found in food. Both are converted to active 5-methyltetrahydrofolate in the body.
Does high folate mask B12 deficiency?
Yes — this is clinically important. High-dose folate supplementation can correct the anaemia of B12 deficiency but leave the neurological damage untreated. Always check both B12 and folate together.
Medical Disclaimer: This page is for general education only and does not replace professional medical advice. Always consult a qualified healthcare provider.