Blood Test

Anti-TPO Antibody Test — Normal Range & Thyroid Autoimmunity

What anti-TPO antibodies are, normal reference ranges, and what a positive test means for your thyroid.

What Is Anti-TPO?

Thyroid peroxidase (TPO) is an enzyme involved in making thyroid hormone. Anti-TPO antibodies are produced by the immune system mistakenly attacking the thyroid gland — a hallmark of autoimmune thyroid disease.

Normal Anti-TPO Range

ResultRange
Negative (normal)Below 35 IU/mL
Positive (elevated)35 IU/mL and above

What a Positive Result Means

Hashimoto's Thyroiditis

The most common cause of an underactive thyroid (hypothyroidism) in adults — anti-TPO is positive in roughly 90–95% of cases.

Graves' Disease

An autoimmune cause of overactive thyroid (hyperthyroidism); anti-TPO can also be positive, alongside TSI/TRAb antibodies.

When Is It Tested?

Important: A positive anti-TPO test does not always mean active thyroid disease — your doctor will interpret it alongside TSH, T3 and T4 results.