Lab Test

Cortisol Blood Test: What Your Levels Mean

Cortisol is produced by the adrenal glands and regulates stress, metabolism, and immune function. Abnormal levels point to conditions like Cushing's syndrome or Addison's disease.

Normal (9am)
140–700 nmol/L
Normal (midnight)
<50 nmol/L (should be low)
Diurnal variation
High in morning, low at night
Stimulation test
Used for Addison's

What Does Cortisol Do?

Interpreting Results

ResultLikely CauseNext Step
High morning cortisolStress, Cushing's syndrome, exogenous steroids24-hour urine free cortisol, dexamethasone suppression test
Low morning cortisolAddison's disease, pituitary failure (secondary adrenal insufficiency)Short Synacthen test (SST)
Loss of diurnal variationCushing's syndrome (midnight cortisol stays high)Late-night salivary cortisol x2
Normal AM cortisol despite symptomsFalse negative — SST may still reveal impaired reserveClinical assessment + SST
Timing Is CriticalCortisol must be taken at 8–9am for meaningful interpretation. A random sample has limited value unless it's very low (<100 nmol/L suggests adrenal insufficiency) or very high.

Cushing's Syndrome

Caused by excess cortisol — either from a pituitary tumour (Cushing's disease), adrenal tumour, or exogenous steroids. Features: central obesity, purple striae, moon face, easy bruising, hypertension, diabetes.

What causes high cortisol?
Chronic stress, obesity, depression, alcohol excess, Cushing's syndrome (pituitary or adrenal tumour), or long-term steroid medication use.
What is Addison's disease?
Autoimmune destruction of the adrenal cortex causing insufficient cortisol and aldosterone. Features: fatigue, weight loss, postural hypotension, bronze skin pigmentation, salt craving, low sodium.
What is the Short Synacthen Test?
A dynamic test that gives synthetic ACTH (synacthen) and measures cortisol at 0 and 30 minutes. A rise to >430 nmol/L rules out primary adrenal insufficiency.
Can cortisol levels be affected by stress?
Yes — acute physical or psychological stress significantly elevates cortisol. Results should be interpreted in the context of the patient's clinical state.
Medical Disclaimer: This page is for general education only and does not replace professional medical advice. Always consult a qualified healthcare provider.